User Guides

Do you offer columns in a 50cm format?


Our column dimensions have been optimised specifically for our 1.6 μm packing material. Due to a combination of unique C18 surface chemistry and bead size, our 25 cm columns consistently outperform larger bead sizes (1.9 μm) used in self-packed 50 cm columns (See our results here). The reduced column volume also requires less re-equilibration time and allows for shorter loading times with higher flow-rates, facilitating much higher throughput of samples.

Our 25 cm Aurora series columns are used exclusively across all the instruments in our own laboratory (Thermo Q-Exactive HF-X, Thermo Q-Exactive, Bruker timsTOF Pro, Bruker Impact II and Bruker Maxis II ETD), with great results time after time.

We’re actively developing a range of new column configurations including different lengths and packing materials. Get in touch via info@ionopticks.com to make sure we keep you up to date.




What LC-MS instruments are Aurora columns compatible with?


We currently offer two column options:

  • Aurora Series Emitter Column (Part No. AUR-25075C18A) - compatible with a column oven (Sonation Column Oven recommended) in an open ion source (e.g. Thermo Nanospray Flex)
  • Aurora Series Emitter Column with CSI fitting (Part No. AUR-25075C18A-CSI) - compatible with a column oven (Sonation Column Oven recommended) secured to a Bruker CaptiveSpray ion source. This model has a pre-fitted CaptiveSpray capillary holder and directly interfaces with the CaptiveSpray ion source with no additional fittings required.

Aurora columns are compatible with the following systems:

UHPLC

  • Bruker (nanoElute)
  • Thermo (EASY-nLC 1000, EASY-nLC 1200, Dionex UltiMateTM)
  • Waters (nanoAcquity, M-Class)

Mass spectrometers:

  • Bruker (with Sonation-compatible captive spray source and Sonation column oven heater)
  • Thermo (with Nanospray Flex Ion Source and Sonation column oven heater)

We use our columns exclusively across all the instruments in our laboratory which includes the above systems. Please contact us via info@ionopticks.com to confirm compatibility with other instruments not listed above.




Can I use Aurora columns with a trapping column?


AURORA columns are designed for the highest possible performance and are optimised for use in a single column configuration (direct injection). The low retentivity of our packing material is not compatible with any commercially available trapping column. Therefore the use of a trapping column is not recommended and will significantly reduce performance.




Which column should I order?


We currently offer two column options:

  • Aurora Series Emitter Column (Part No. AUR-25075C18A) - compatible with a column oven (Sonation Column Oven recommended) in an open ion source (e.g. Thermo Nanospray Flex)
  • Aurora Series Emitter Column with CSI fitting (Part No. AUR-25075C18A-CSI) - compatible with a column oven (Sonation Column Oven recommended) secured to a Bruker CaptiveSpray ion source. This model has a pre-fitted CaptiveSpray capillary holder and directly interfaces with the CaptiveSpray ion source with no additional fittings required.

Please contact us via info@ionopticks.com if you would like to confirm your compatibility requirements.




What options do you offer regarding packing materials?


We currently offer AURORA columns packed with C18 silica resin (1.6um, 120Å pore size).

We welcome inquiries regarding alternate packing materials. Please contact info@ionopticks.com to discuss your requirements.

We’re actively developing a range of new column configurations including different lengths and packing materials. Get in touch so we can keep you up to date.




What additional fittings and adapters do I need?


Our columns are pre-fitted with our nanoZero™ fitting that provides a true zero-dead-volume female union between a nanoViper™ Fingertight fitting (or equivalent) and the Aurora series column. No additional fittings are required.




Do I need a column oven/heater?


Yes! Our columns require a column oven installed at the mass spectrometer ion source (we recommend Sonation Column Oven PRSO-V1/PRSO-V2). Operating a column without a column oven will result in overpressuring at optimal flow rates and fluctuating operating temperatures generating inconsistent sample separation.




What temperature should Aurora columns be operated at?


We recommend operating Aurora columns at 40-50°C for optimal performance. The maximum operating temperature is 60°C. At room temperature, the column itself will not be harmed but will result in increased UHPLC back pressure and temperature fluctuations which can make results more inconsistent than if the column was run at a stable temperature in a column oven.

Operation of Aurora columns at room temperature will result in sub-optimal flow velocities and poor performance.




How should I prepare my samples for a single column (direct injection) configuration?


Samples loaded on to the column should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Our Lab uses neutral SP3 or FASP assited digestion and de-salts with C18 Stagetips for great results (see Sielaff M et al. Journal of proteome research. 2017). Using these methods we obtain a column lifespan of 300+ injections. Loading contaminated samples onto the column may disrupt solvent flow or foul the emitter tip leading to a loss of performance. For further details on recommended sample preparation protocols, please contact us at support@ionopticks.com.




What flow rates should be used?


For typical operation of the columns we recommend a flow rate around 400 nl/min with the column oven set to 40ºC. This will ensure UHPLC backpressures of around 700-800bar. Flow rates can be increased/decreased to suit particular applications.




Do you make custom columns to spec?


We welcome inquiries regarding alternate packing materials and column lengths. Please contact support@ionopticks.com to discuss your requirements.




How do I order?


Great news! Please contact us at sales@ionopticks.com and we can provide you with a quote.




Do you ship internationally?


All columns are manufactured and packed in Melbourne, Australia and shipped globally. For all international orders, we prefer to use FedEx to make sure our products arrive as soon as possible. We can provide a FedEx shipping estimate with your initial quote, or alternatively, use your organisation’s account to ship the goods. Simply tell us your preferred option when placing your order.




What about Customs Duties and Taxes?


Our prices exclude shipping as well as duties and taxes, which may be payable at the receiving port.

Please note that shipping, duties and customs taxes are payable by the purchaser on all orders.




How long will it take for me to receive my order?


We will confirm receipt of your order within 24 hours and confirm when the order will be shipped. Once shipped, typical shipping times are:

  • 1-2 business days for Australian customers
  • 3-5 business days for international customers, subject to customs clearance.




What if there is a fault with my column?


Our columns are subjected to rigorous quality control procedures under the direct eye of our Senior Scientists. A comprehensive user guide is also provided with each column to assist with implementation of the column. However, should you encounter any issues, we offer a 100% replacement guarantee on manufacturing faults.

Please contact us at support@ionopticks.com if you have any concerns relating to your column. Our detailed terms and conditions are provided with every quote but we want to make sure you are receiving a quality product every time so please do not hesitate to get in touch with any feedback or concerns.




How will my order be shipped?


All columns are manufactured and packed in Melbourne, Australia and shipped globally. For Australian orders, your order will be shipped via DHL or Australia Post (Express) and should arrive within 1-2 days of being sent. All orders require a signature on receipt. For international orders, we currently ship with FedEx to make sure our products arrive as soon as possible. We can provide a FedEx shipping estimate with your initial quote, or alternatively, use your organisation’s account to ship the goods. Simply tell us your preferred option when placing your order. We are also working on other ways to get our columns to you to make the process even easier.




What if my order is delayed at customs?


You will be notified as soon as your order is shipped and provided with a tracking number. If you experience any delays please contact us at sales@ionopticks.com so we can assist with providing further information to customs as necessary.




How long can I store my column for?


Aurora Series columns should be stored in a cool dry environment. Each column has a recommended shelf life of 24 months from the date of manufacture, making them easy and cost effective to buy in bulk and store in your lab ready for use.




Are your columns RoHS compliant?


Yes. Please contact support@ionopticks.com if you require further details.





 

Please contact support@ionopticks.com if you require further assistance

 

Frequently Asked Questions

Do you offer columns in a 50cm format?


Our column dimensions have been optimised specifically for our 1.6 μm packing material. Due to a combination of unique C18 surface chemistry and bead size, our 25 cm columns consistently outperform larger bead sizes (1.9 μm) used in self-packed 50 cm columns (See our results here). The reduced column volume also requires less re-equilibration time and allows for shorter loading times with higher flow-rates, facilitating much higher throughput of samples.

Our 25 cm Aurora series columns are used exclusively across all the instruments in our own laboratory (Thermo Q-Exactive HF-X, Thermo Q-Exactive, Bruker timsTOF Pro, Bruker Impact II and Bruker Maxis II ETD), with great results time after time.

We’re actively developing a range of new column configurations including different lengths and packing materials. Get in touch via info@ionopticks.com to make sure we keep you up to date.




What LC-MS instruments are Aurora columns compatible with?


We currently offer two column options:

  • Aurora Series Emitter Column (Part No. AUR-25075C18A) - compatible with a column oven (Sonation Column Oven recommended) in an open ion source (e.g. Thermo Nanospray Flex)
  • Aurora Series Emitter Column with CSI fitting (Part No. AUR-25075C18A-CSI) - compatible with a column oven (Sonation Column Oven recommended) secured to a Bruker CaptiveSpray ion source. This model has a pre-fitted CaptiveSpray capillary holder and directly interfaces with the CaptiveSpray ion source with no additional fittings required.

Aurora columns are compatible with the following systems:

UHPLC

  • Bruker (nanoElute)
  • Thermo (EASY-nLC 1000, EASY-nLC 1200, Dionex UltiMateTM)
  • Waters (nanoAcquity, M-Class)

Mass spectrometers:

  • Bruker (with Sonation-compatible captive spray source and Sonation column oven heater)
  • Thermo (with Nanospray Flex Ion Source and Sonation column oven heater)

We use our columns exclusively across all the instruments in our laboratory which includes the above systems. Please contact us via info@ionopticks.com to confirm compatibility with other instruments not listed above.




Can I use Aurora columns with a trapping column?


AURORA columns are designed for the highest possible performance and are optimised for use in a single column configuration (direct injection). The low retentivity of our packing material is not compatible with any commercially available trapping column. Therefore the use of a trapping column is not recommended and will significantly reduce performance.




Which column should I order?


We currently offer two column options:

  • Aurora Series Emitter Column (Part No. AUR-25075C18A) - compatible with a column oven (Sonation Column Oven recommended) in an open ion source (e.g. Thermo Nanospray Flex)
  • Aurora Series Emitter Column with CSI fitting (Part No. AUR-25075C18A-CSI) - compatible with a column oven (Sonation Column Oven recommended) secured to a Bruker CaptiveSpray ion source. This model has a pre-fitted CaptiveSpray capillary holder and directly interfaces with the CaptiveSpray ion source with no additional fittings required.

Please contact us via info@ionopticks.com if you would like to confirm your compatibility requirements.




What options do you offer regarding packing materials?


We currently offer AURORA columns packed with C18 silica resin (1.6um, 120Å pore size).

We welcome inquiries regarding alternate packing materials. Please contact info@ionopticks.com to discuss your requirements.

We’re actively developing a range of new column configurations including different lengths and packing materials. Get in touch so we can keep you up to date.




What additional fittings and adapters do I need?


Our columns are pre-fitted with our nanoZero™ fitting that provides a true zero-dead-volume female union between a nanoViper™ Fingertight fitting (or equivalent) and the Aurora series column. No additional fittings are required.




Do I need a column oven/heater?


Yes! Our columns require a column oven installed at the mass spectrometer ion source (we recommend Sonation Column Oven PRSO-V1/PRSO-V2). Operating a column without a column oven will result in overpressuring at optimal flow rates and fluctuating operating temperatures generating inconsistent sample separation.




What temperature should Aurora columns be operated at?


We recommend operating Aurora columns at 40-50°C for optimal performance. The maximum operating temperature is 60°C. At room temperature, the column itself will not be harmed but will result in increased UHPLC back pressure and temperature fluctuations which can make results more inconsistent than if the column was run at a stable temperature in a column oven.

Operation of Aurora columns at room temperature will result in sub-optimal flow velocities and poor performance.




How should I prepare my samples for a single column (direct injection) configuration?


Samples loaded on to the column should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Our Lab uses neutral SP3 or FASP assited digestion and de-salts with C18 Stagetips for great results (see Sielaff M et al. Journal of proteome research. 2017). Using these methods we obtain a column lifespan of 300+ injections. Loading contaminated samples onto the column may disrupt solvent flow or foul the emitter tip leading to a loss of performance. For further details on recommended sample preparation protocols, please contact us at support@ionopticks.com.




What flow rates should be used?


For typical operation of the columns we recommend a flow rate around 400 nl/min with the column oven set to 40ºC. This will ensure UHPLC backpressures of around 700-800bar. Flow rates can be increased/decreased to suit particular applications.




Do you make custom columns to spec?


We welcome inquiries regarding alternate packing materials and column lengths. Please contact support@ionopticks.com to discuss your requirements.




How do I order?


Great news! Please contact us at sales@ionopticks.com and we can provide you with a quote.




Do you ship internationally?


All columns are manufactured and packed in Melbourne, Australia and shipped globally. For all international orders, we prefer to use FedEx to make sure our products arrive as soon as possible. We can provide a FedEx shipping estimate with your initial quote, or alternatively, use your organisation’s account to ship the goods. Simply tell us your preferred option when placing your order.




What about Customs Duties and Taxes?


Our prices exclude shipping as well as duties and taxes, which may be payable at the receiving port.

Please note that shipping, duties and customs taxes are payable by the purchaser on all orders.




How long will it take for me to receive my order?


We will confirm receipt of your order within 24 hours and confirm when the order will be shipped. Once shipped, typical shipping times are:

  • 1-2 business days for Australian customers
  • 3-5 business days for international customers, subject to customs clearance.




What if there is a fault with my column?


Our columns are subjected to rigorous quality control procedures under the direct eye of our Senior Scientists. A comprehensive user guide is also provided with each column to assist with implementation of the column. However, should you encounter any issues, we offer a 100% replacement guarantee on manufacturing faults.

Please contact us at support@ionopticks.com if you have any concerns relating to your column. Our detailed terms and conditions are provided with every quote but we want to make sure you are receiving a quality product every time so please do not hesitate to get in touch with any feedback or concerns.




How will my order be shipped?


All columns are manufactured and packed in Melbourne, Australia and shipped globally. For Australian orders, your order will be shipped via DHL or Australia Post (Express) and should arrive within 1-2 days of being sent. All orders require a signature on receipt. For international orders, we currently ship with FedEx to make sure our products arrive as soon as possible. We can provide a FedEx shipping estimate with your initial quote, or alternatively, use your organisation’s account to ship the goods. Simply tell us your preferred option when placing your order. We are also working on other ways to get our columns to you to make the process even easier.




What if my order is delayed at customs?


You will be notified as soon as your order is shipped and provided with a tracking number. If you experience any delays please contact us at sales@ionopticks.com so we can assist with providing further information to customs as necessary.




How long can I store my column for?


Aurora Series columns should be stored in a cool dry environment. Each column has a recommended shelf life of 24 months from the date of manufacture, making them easy and cost effective to buy in bulk and store in your lab ready for use.




Are your columns RoHS compliant?


Yes. Please contact support@ionopticks.com if you require further details.





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Copyright 2020. Patents Pending. All Rights Reserved.  

Ion Opticks Pty Ltd  ABN 99 621 674 459

Parkville, Victoria, Australia

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