Ion Mobility assisted precursor selection benefits Cross-linking Mass Spectrometry.
Chemical cross-linking of interacting proteins locks them in place, and when combined with MS based proteomics, facilitates the identification of tertiary and quaternary structural information. This powerful approach allows researchers to better understand how proteins affect biological processes such as complex formation, signal transduction and enzymatic regulation, as nearly all protein functions are a consequence of their interactions.
Using IonOpticks Aurora Series columns, Steigenberger et al. at Utrecht University demonstrate the advantages of using trapped ion mobility to separate the inherent differences in collisional cross sectional area of mono-linked and cross-linked peptides. This exclusion of peptides with limited cross-linking information led to a substantial increase in detected cross-linked peptides.
Benefits of Collisional Cross Section Assisted Precursor Selection (caps-PASEF) for Cross-linking Mass Spectrometry. bioRxiv. 2020 Apr 18. doi: https://doi.org/10.1101/2020.04.16.044859