• Andrew Webb

Aurora columns advance Single Cell Proteomics

Updated: Jan 14

The study of single cell -omics has generated enormous interest in the scientific field over the last few years. The analysis of complex biological systems at the single cell level provides insights into fundamental biological phenomena that were either hidden or only partly discernible using previous approaches that average data across a population of cells.

Until recently, single cell analysis of proteins has been limited to immunofluorescent techniques and has remained out of reach of broad discovery methods such as mass spectrometry.

Nikolai Slavov, an Assistant Professor at Northeastern University, has developed a TMT-based approach to Single Cell Proteomics by Mass Spectrometry (SCoPE-MS) that is made possible using IonOpticks Aurora series columns. In his most recent work, they report the SCoPE2 methodology that substantially increases the capabilities of the original SCoPE method, reducing cost and analysis time.

In this recent genome webinar, Harrison Specht from the Slavov Lab describes how SCoPE2 lowers cost and hands-on time for proteomic analysis of single cells. Hear how this was achieved using only commercially available equipment and reagents with IonOpticks columns here.

Single-cell mass-spectrometry quantifies the emergence of macrophage heterogeneity.

bioRxiv doi: https://doi.org/10.1101/665307 Specht H, Emmott E, Petelski AA, Huffman RG, Perlman DH, Serra M, Kharchenko P, Koller A, Slavov N.