• Andrew Webb

A new sensitive and accurate method enables endogenous phosphotyrosine mapping

Updated: Jan 14

Phosphotyrosine (pTyr) plays a central role in many cell-to-cell communication pathways, including those that regulate proliferation, differentiation, adhesion, hormone responses, and immune defense. These pathways are commonly dysregulated in cancer and have provided attractive targets for therapeutic intervention.

Historically, measurement of global changes of pTyr has predominantly been limited to model systems, requiring large amounts of input material due the low abundant nature of these modifications. In this recent BioRxiv publication, Stopfer and colleagues demonstrate a workflow, termed “SureQuant pTyr”, that can reliably and accurately quantify hundreds of endogenous pTyr targets.

The plug and play nature of IonOpticks Aurora series columns helped in facilitating the turn-key nature of this approach that opens the door to rapid and accurate profiling in clinical research settings.

High-density, targeted monitoring of tyrosine phosphorylation reveals activated signaling networks in human tumors. BioRxiv. 2020 Jun 2. doi: https://doi.org/10.1101/2020.06.01.127787

Stopfer LE, Flower CT, Gajadhar AS, Patel B, Gallien S, Lopez-Ferrer D, White FM.